General Information

  • ID:  hor001647
  • Uniprot ID:  Q8JIM3
  • Protein name:  Growth hormone-releasing peptide
  • Gene name:  NA
  • Organism:  Lithobates catesbeianus (American bullfrog) (Rana catesbeiana)
  • Family:  FARP (FMRFamide related peptide) family
  • Source:  Animal
  • Expression:  Observed in the suprachiasmatic nucleus and in several telencephalic and diencephalic regions.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Lithobates (genus), Ranidae (family), Ranoidea (superfamily), Neobatrachia (suborder), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003674 molecular_function
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0046879 hormone secretion
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  SLKPAANLPLRF
  • Length:  12(96-107)
  • Propeptide:  MHLKIGTLTIRTIMLFTLCTFLTLFAFSTCFDETKTINLESQEIYDDLFESKEDLQNERNGNSDEYRYMGSNSINDINRYNRLSMQPENEFLQERSLKPAANLPLRFGRTSDDKIAKSIPSFDKIAKSIPNLPQRFGRYLSGKTKVQSMANLPQRFGRAQYTNHFVHSLDTLPLRFGRTPHSDRLQYEMNSHPLELKNPEEDSDRKKRQAMTFRIRTDLQM
  • Signal peptide:  MHLKIGTLTIRTIMLFTLCTFLTLF
  • Modification:  T12 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Primary role is to release GH from the pituitary. May act as an endogenous ligand in the bullfrog hypothalamo-hypophysial system.
  • Mechanism:  NA
  • Cross BBB:  YES
  • Target:  NA
  • Target Unid:   NA
  • IC50:  NA
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q8JIM3-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor001647_AF2.pdbhor001647_ESM.pdb

Physical Information

Mass: 152299 Formula: C62H103N17O15
Absent amino acids: CDEGHIMQTVWY Common amino acids: L
pI: 11.65 Basic residues: 2
Polar residues: 2 Hydrophobic residues: 6
Hydrophobicity: 15.83 Boman Index: -915
Half-Life / Aliphatic Index: 1.9 hour Aliphatic Index: 114.17
Instability Index: 4395.83 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  11796493
  • Title:  A novel amphibian hypothalamic neuropeptide: isolation, localization, and biological activity.